Titan Krios is a state-of-the-art (300kV) cryogenic transmission electron microscope that enables high-resolution structure visualization via single-particle electron microscopy as well as electron tomography.
Krios is dedicated primarily for imaging specimens that have either potential for yielding high-resolution reconstructions (e.g., preliminary reconstruction showing visible secondary structure) and for specimens that can only be imaged on the Krios (e.g. thickness requiring 300 kV and energy filtering).
- 300 kV X-FEG
- Autoloader - up to 12 grids can be loaded simultaneously under cryogenic conditions
- K3 Gatan Direct Detector
- BioQuantum Gatan Image Filter
- Ceta 16M Camera
- Falcon 3EC Detector
- Parallel Illumination
- Phase Plate
- Dual axis tilt holder (+/- 70 degrees)
- Automated data collection
- High-Resolution Imaging of vitrified biological or beam sensitive materials
- Cryo-Electron Tomography
MIT.nano (basement level)
60 Vassar Street (rear)
Charged hourly with the expectation of 24-hr sessions.
Before scheduling data collection, specimens should be registered on a MIT BRR and screened for appropriate blotting conditions, sample concentration, and homogeneity.