MIT.nano CryoEM sample registration

General Information

You must set up a MUMMS account to use the CryoEM Facility (https://nanolms.mit.edu/mumms/registration.html).

Pre-screening

Have you previously registered this specimen material and its components for use in the cryo-EM facility?
The sample name must match a previously registered sample.
This should be a unique identifyer for your sample. You will be prompted to include a more thorough sample description in the next question.
For this sample, I would like to use the facility for:
One file only.
20 MB limit.
Allowed types: txt, rtf, pdf, doc, docx, odt, ppt, pptx, odp, xls, xlsx, ods.

Thank you for already registering your sample!

After you receive sample approval, please proceed to the CryoEM session request form: https://mitnanouser.mit.edu/mitnano-cryogenic-electron-microscopy-session-request
Please indicate your availability for a session
I understand that checking this box indicates that I have answered all of the above questions to the best of my knowledge and that I agree to only bring properly approved materials into MIT.nano. I will follow all policies and procedures outlined in MIT.nano documentation and training. If found out of compliance, I understand this may result in loss of access to MIT.nano facilities.

Sample Information

Use this form for each new sample you would like to bring into the CryoEM facility for specimen preparation and/or imaging (Bioprep Lab, Aquilos, Talos Arctica, Titan Krios). To register multiple samples you will need to submit the form more than once.
This should be a unique identifyer for your sample. You will be prompted to include a more thorough sample description in the next question.
Be descriptive (e.g. organism, species and genus, strain, variant, protein name, cell line, expression source, non-biological organic or inorganic material, etc.). For example, human XYZ complex extracted from E. coli, S. cerevisiae transformed with pRS415MET25-GFP, lead sulfide nanoparticles with oleic acid surfactant.
Will your sample already frozen on a TEM grid prior to bringing it into the MIT.nano CryoEM facility?
Is your sample biological, derived/isolated from a biological system (e.g. cell or organism), or recombinant or synthetic nucleic acid molecules (e.g. DNA/RNA)?

Biological Sample Information

Which categories best describe your sample?
Please select all that apply.
Is your sample purified or crude?
Is your sample biologically inactivated and poses no hazards to personnel and the environment?
Please select the method used to inactivate the specimen.
Are you an internal or external user?
Is this biological sample or research registered with the MIT CAB/ESCRO?
The Committee on Assessment of Biohazards and Embryonic Stem Cell Research Oversight (CAB/ESCRO) ensures the safe and responsible conduct of biological research at MIT. Visit the CAB/ESCRO website or contact your Biosafety Officer to determine if your sample or research requires registration (https://ehs.mit.edu/about/institute-committees/cab-escro-committee/).
Is this biological sample or research registered with the MIT CAB/ESCRO through a MIT collaborator's Biological Research Registration (BRR)?
The Committee on Assessment of Biohazards and Embryonic Stem Cell Research Oversight (CAB/ESCRO) ensures the safe and responsible conduct of biological research at MIT. Visit the CAB/ESCRO website to determine if your sample or research falls within the purview of the CAB/ESCRO (https://ehs.mit.edu/about/institute-committees/cab-escro-committee/).
Contact your MIT Biosafety Officer if you do not know your lab's BRR number.

Biological Research Registration (BRR) is required.

Please contact your Biosafety Officer to assist with the BRR process. Your sample registration for the MIT.nano CryoEM Facility will remain under review until you have received CAB/ESCRO approval to work with this biological material.

Biological Research Registration (BRR) is required.

If your biological sample is not already registered on MIT.nano's BRR, we will work with you to add it to our BRR for CAB/ESCRO review. Your sample registration for the MIT.nano CryoEM Facility will remain under review until we have received CAB/ESCRO approval to work with this biological material.
Have your collaborator contact their MIT Biosafety Officer if you they do not know the lab's BRR number.
Which of the following describes your sample?
If available, attach data sheets for your specimen with any test data (e.g. certificate of analysis) for bloodborne pathogens and other documentation that may assist in the analysis.
One file only.
20 MB limit.
Allowed types: jpg, jpeg, png, txt, rtf, html, odf, pdf, doc, docx, ppt, pptx, xls, xlsx, xml, avi, mov, mp3, ogg, wav, bz2, dmg, gz, jar, rar, sit, svg, tar, zip.

Additional Sample Information

We will work with you to establish procedures for your specimen that ensure safety for all users of the facility.
Is your sample a nanomaterial or contain nanomaterials (material with at least one dimension <100 nm)?
Which additional hazards are not described in the above questions?
Be descriptive. Provide enough information to permit reasonable understanding by a lay person & to allow for risk assessment.
Select method(s) of decontamination and/or disinfection appropriate for your sample.
Please select all that apply.
Select method(s) of waste disposal appropriate for your sample.
Please select all that apply.

I understand that checking this box indicates that I have answered all of the above questions to the best of my knowledge and that I agree to only bring properly approved materials into MIT.nano. I will follow all policies and procedures outlined in MIT.nano documentation and training. If found out of compliance, I understand this may result in loss of access to MIT.nano facilities.