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Titan Krios G3i Cryo-TEM

Titan Krios is a state-of-the-art (300kV) cryogenic transmission electron microscope that enables high-resolution structure visualization via single-particle electron microscopy as well as electron tomography.

Krios is dedicated primarily for imaging specimens that have either potential for yielding high-resolution reconstructions (e.g., preliminary reconstruction showing visible secondary structure) and for specimens that can only be imaged on the Krios (e.g. thickness requiring 300 kV and energy filtering).

Specifications/Capabilities
  • 300 kV X-FEG
  • Autoloader - up to 12 grids can be loaded simultaneously under cryogenic conditions 
  • K3 Gatan Direct Detector
  • BioQuantum Gatan Image Filter
  • Ceta 16M Camera
  • Falcon 3EC Detector
  • Parallel Illumination
  • Phase Plate
  • Dual axis tilt holder (+/- 70 degrees)
  • Automated data collection
Applications
  • High-Resolution Imaging of vitrified biological or beam sensitive materials
  • Cryo-Electron Tomography 
Location

12-0175
MIT.nano (basement level)
60 Vassar Street (rear)
Cambridge, MA

Reservation Rules

Before scheduling collection, specimens should be screened for appropriate blotting conditions, sample concentration, and homogeneity.

Staff/Contact

Ed Brignole, PhD
Department of Biology
Assistant Director CryoEM

Phat Dip, PhD
Department of Biology
Research Scientist


12-0174
MIT.nano (basement level)
60 Vassar Street (rear)
Cambridge, MA

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